![]() ![]() Can I pulldown a HaloTag fusion protein, that is already bound to a substrate?.How can I immunoprecipitate my Halo-tagged protein?.How can I detect my Halo-tagged protein in Western blot?.What are the differences between HaloTag and GFP?.What are the differences between HaloTag and Snap-Tag?.Is there more than one HaloTag version available?.For what applications can HaloTag be used?.How is the complex between the HaloTag and a ligand formed?.Sticky ends from different EcoO109I sites may not be compatible. Sticky ends from different BlpI sites may not be compatible. Sticky ends from different BtgI sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. PaeR7I does not recognize the sequence CTCTCGAG. Sticky ends from different BbsI sites may not be compatible.ībsI gradually loses activity when stored at -20☌.īssHII is typically used at 50☌, but is 75% active at 37☌. PshAI quickly loses activity at 37☌, but can be used at 25☌ for long incubations.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different PasI sites may not be compatible.īclI is typically used at 50-55☌, but is 50% active at 37☌. This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. Sticky ends from different Tth111I sites may not be compatible. ![]()
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